Hurd E R, Hashimoto Y
Inflammation. 1981 Sep;5(3):213-22. doi: 10.1007/BF00914445.
Using a Coulter counter method, the effects of various types of IgG-dependent phagocytic stimuli on human neutrophil (PMN) swelling were determined. Human heat aggregated IgG, ovalbumin-antiovalbumin (OV-anti-OV) immune complexes, and opsonized latex particles all induced PMN swelling. The OV-anti-OV immune complexes were effective, whether prepared at antigen-antibody equivalence (insoluble) or at 4 or 9 times antigen excess (soluble). Swelling of PMN occurred at 37 degrees C, but not at 4 degrees C. Complement was not present in any of the experiments. In contrast to the above results, native IgG, OV-anti-OV F(ab')2 immune complexes and unopsonized latex particles did not induce PMN swelling. These results suggest that the PMN swelling observed in this study is due to Fc-dependent, complement-independent membrane stimulation and/or phagocytosis.
采用库尔特计数法，测定了各种类型的IgG依赖性吞噬刺激对人中性粒细胞（PMN）肿胀的影响。人热聚集IgG、卵清蛋白 - 抗卵清蛋白（OV - 抗 - OV）免疫复合物和调理化乳胶颗粒均诱导PMN肿胀。无论在抗原 - 抗体等价（不溶性）时制备，还是在抗原过量4倍或9倍（可溶性）时制备，OV - 抗 - OV免疫复合物均有效。PMN肿胀发生在37℃，而不是4℃。所有实验中均不存在补体。与上述结果相反，天然IgG、OV - 抗 - OV F(ab')2免疫复合物和未调理化乳胶颗粒不诱导PMN肿胀。这些结果表明，本研究中观察到的PMN肿胀是由于Fc依赖性、补体非依赖性的膜刺激和/或吞噬作用。
