Binns R M, Blakeley D, Licence S T
Int Arch Allergy Appl Immunol. 1981;66(3):341-9. doi: 10.1159/000232839.
Optimum conditions were determined for FITC and RITC labelling of pig blood lymphocytes with bright fluorescence and high viability and for their enumeration during their long survival after infusion in vivo. For at least 9 days, FITZ-labelled autologous lymphocytes and lymphocytes from MHC-identical donors showed no detectable difference in patterns of survival in the blood. After initial equilibration with extra-vascular lymphoid tissues, the fall-off suggested the expected presence of populations with a variety of life-spans, with some autologous labelled lymphocytes surviving beyond 3 weeks. A trace of cytotoxic antibody specific for labelled cells developed in these pigs later and could be boosted by a further FITC-labelled cell infusion.
确定了用异硫氰酸荧光素（FITC）和四甲基异硫氰酸罗丹明（RITC）标记猪血液淋巴细胞的最佳条件，使其具有明亮的荧光和高活力，并在体内输注后长时间存活期间对其进行计数。在至少9天的时间里，FITC标记的自体淋巴细胞和来自主要组织相容性复合体（MHC）相同供体的淋巴细胞在血液中的存活模式没有可检测到的差异。在与血管外淋巴组织初步平衡后，细胞数量的下降表明预期存在具有不同寿命的群体，一些自体标记的淋巴细胞存活超过3周。这些猪后来产生了微量针对标记细胞的细胞毒性抗体，进一步输注FITC标记的细胞可增强这种抗体。
