Rothkötter H J, Huber T, Barman N N, Pabst R
Centre of Anatomy, Medical School of Hannover, Germany.
Clin Exp Immunol. 1993 May;92(2):317-22. doi: 10.1111/j.1365-2249.1993.tb03398.x.
Gut wall emigrating cells have been characterized in the intestinal lymph. The intestinal lymph duct was cannulated in 6-month-old minipigs. Under non-restraining conditions the efferent lymph from the mesenteric lymph nodes was collected in seven normal animals. Lymph coming directly from the gut (afferent lymph) was also collected in 18 pigs after resection of the mesenteric lymph node chains 3 months previously. The intestinal lymph flow was similar in both groups (around 18 ml/h). The lymphoid cell yield was 1.2 +/- 1.0 x 10(6)/h in control animals, while in mesenteric lymph node resected pigs it was around 20 times higher (26.2 +/- 17.6 x 10(6)/h). In the gut-derived lymph 76.5 +/- 8.8% T lymphocytes were observed (CD4+, 48.1 +/- 15.5%; CD8+, 53.6 +/- 12.7%). The percentage of immunoglobulin-positive cells was lower (IgM+, 10.1 +/- 4.5; IgA+, 1.7 +/- 1.1). In 14 mesenteric lymph node resected pigs a mean of 5.6 +/- 3.1 x 10(8) lymphocytes from the gut lymph were labelled in vitro with a fluorescent dye and retransfused. The labelling index of fluorescent cells in the intestinal lymph increased rapidly and remained at a high level until 44 h after cell transfusion. A four-to-ten times lower labelling index was found in the spleen, various lymph nodes and Peyer's patches. Most of the recovered lymphocytes were T cells. This model provides access to the cell pool leaving the gut wall, thus allowing an examination of its role in the gastrointestinal tract and other mucosal-lined organs.
肠壁迁移细胞已在肠淋巴中得到鉴定。在6月龄小型猪身上对肠淋巴管进行插管。在非限制条件下，收集了7只正常动物来自肠系膜淋巴结的输出淋巴。在3个月前切除肠系膜淋巴结链后，还收集了18头猪直接来自肠道的淋巴（输入淋巴）。两组的肠淋巴流量相似（约18毫升/小时）。对照动物的淋巴细胞产量为1.2±1.0×10⁶/小时，而在切除肠系膜淋巴结的猪中，产量约高20倍（26.2±17.6×10⁶/小时）。在源自肠道的淋巴中，观察到76.5±8.8%的T淋巴细胞（CD4⁺，48.1±15.5%；CD8⁺，53.6±12.7%）。免疫球蛋白阳性细胞的百分比更低（IgM⁺，10.1±4.5；IgA⁺，1.7±1.1）。在14只切除肠系膜淋巴结的猪中，平均5.6±3.1×10⁸个来自肠道淋巴的淋巴细胞在体外用荧光染料标记后重新输注。肠道淋巴中荧光细胞的标记指数迅速增加，并在细胞输注后44小时内保持在高水平。在脾脏、各种淋巴结和派尔集合淋巴结中发现的标记指数低4至10倍。回收的淋巴细胞大多数是T细胞。该模型提供了进入离开肠壁的细胞池的途径，从而能够研究其在胃肠道和其他有黏膜衬里器官中的作用。
