Górny M K, Jezewska E, Zeromski J
Arch Geschwulstforsch. 1981;51(5):418-23.
A panel of sera derived from 138 patients with primary bronchogenic carcinoma, non-neoplastic lung conditions and from blood donors was tested for presence of autoantibodies by indirect immunofluorescence on fixed cells of established lung cancer cell line and lung fibroblasts as a substrate. Autoantibodies were detected in 87% and 64% out of patient sera respectively and in 9% of donor sera. Immunofluorescence patterns permitted to distinguish 3 antibody specificities: anti-nucleolar, anti-cytoplasmic and anti-nuclear ones. The major differences were noted in incidence of anti-nucleolar antibodies, which were present in 77% of lung cancer patients and only in 14% of patients with non-neoplastic lung conditions. The autoantibodies in question belonged to IgG and to lesser degree IgA class of immunoglobulin and were not apparently cancer specific because absorption with normal tissue homogenates removed their activity.
通过间接免疫荧光法，以已建立的肺癌细胞系和肺成纤维细胞的固定细胞为底物，对一组来自138例原发性支气管肺癌患者、非肿瘤性肺部疾病患者以及献血者的血清进行自身抗体检测。分别在87%的患者血清和64%的患者血清中检测到自身抗体，在9%的献血者血清中也检测到自身抗体。免疫荧光模式可区分3种抗体特异性：抗核仁、抗细胞质和抗核抗体。主要差异在于抗核仁抗体的发生率，77%的肺癌患者存在该抗体，而在非肿瘤性肺部疾病患者中仅为14%。所讨论的自身抗体属于免疫球蛋白的IgG类，IgA类含量较少，且显然并非癌症特异性，因为用正常组织匀浆吸收后会消除其活性。
