乳糜泻的体外诊断：一项评估。
In vitro diagnosis of coeliac disease: an assessment.
作者信息
Howdle P D, Corazza G R, Bullen A W, Losowsky M S
出版信息
Gut. 1981 Nov;22(11):939-47. doi: 10.1136/gut.22.11.939.
Abstract
Jejunal biopsies from controls and coeliac patients were maintained in organ culture for up to 48 hours. The in vitro effect of gluten fraction III during the period of culture was assessed by measurement of the activity of the brush border enzymes alkaline phosphatase and alpha-glucosidase. Mucosa from controls and treated and untreated coeliacs behaved similarly and no reproducible in vitro effect of gluten was demonstrated. These results cast doubt on the in vitro diagnosis of coeliac disease by monitoring brush border enzyme activity.
摘要
将对照组和乳糜泻患者的空肠活检组织在器官培养中保存长达48小时。通过测量刷状缘酶碱性磷酸酶和α-葡萄糖苷酶的活性来评估培养期间麸质组分III的体外作用。对照组以及经过治疗和未经治疗的乳糜泻患者的黏膜表现相似，未显示出麸质具有可重复的体外作用。这些结果对通过监测刷状缘酶活性进行乳糜泻的体外诊断提出了质疑。
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