从粪便中常规分离艰难梭菌的简化程序。
Simplified procedure for the routine isolation of Clostridium difficile from faeces.
作者信息
Borriello S P, Honour P
出版信息
J Clin Pathol. 1981 Oct;34(10):1124-7. doi: 10.1136/jcp.34.10.1124.
Abstract
The use of alcohol, at a final concentration of 50%, as a selective procedure for the isolation of Clostridium difficile was compared to a selective medium containing 250 microgram /ml of cycloserine and 10 microgram /ml of cefoxitin. Of 266 faecal samples 82 were shown to be positive by one or other method. Seventy-seven (94%) of these were detected by the selective agar (SA) and 72 (88%) by the alcohol procedure (AP). Ten samples (12%) were positive only by SA and five samples (6%) by AP only. The AP was further modified so that all manipulations prior to incubation were performed on the open bench. Of 18 positive samples, 18 (100%) were detected by SA and 16 (89%) by AP.
摘要
将终浓度为50%的酒精用作艰难梭菌分离的选择方法，并与含有250微克/毫升环丝氨酸和10微克/毫升头孢西丁的选择培养基进行比较。在266份粪便样本中，有82份通过其中一种方法显示为阳性。其中77份（94%）通过选择琼脂（SA）检测到，72份（88%）通过酒精法（AP）检测到。10份样本（12%）仅通过SA呈阳性，5份样本（6%）仅通过AP呈阳性。对AP进行了进一步改良，以便在孵育前的所有操作都在开放实验台上进行。在18份阳性样本中，18份（100%）通过SA检测到，16份（89%）通过AP检测到。
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