Kloner R A, DeBoer L W, Darsee J R, Ingwall J S, Braunwald E
Proc Natl Acad Sci U S A. 1981 Nov;78(11):7152-6. doi: 10.1073/pnas.78.11.7152.
The purpose of this study was to determine whether or not the biochemical, functional, and ultrastructural abnormalities produced by brief temporary coronary occlusions (unassociated with necrosis) ever resolve and, if so, when they do. Anesthetized open-chest dogs were subjected to 15 min of coronary artery occlusion followed by 72 hr, 7 days, or 14 days of reperfusion. Serial in vivo myocardial biopsies were performed for measurement of ATP and for ultrastructural analysis. Regional function was evaluated by sonomicrometry. Mean (+/- SEM) myocardial ATP concentration was 36.6 +/- 1.2 nmol/mg of cardiac protein in nonischemic subendocardium and 18.9 +/- 1.5 in ischemic subendocardium after 15 min of ischemia. ATP remainede performed for measurement of ATP and for ultrastructural analysis. Regional function was evaluated by sonomicrometry. Mean (+/- SEM) myocardial ATP concentration was 36.6 +/- 1.2 nmol/mg of cardiac protein in nonischemic subendocardium and 18.9 +/- 1.5 in ischemic subendocardium after 15 min of ischemia. ATP remainede performed for measurement of ATP and for ultrastructural analysis. Regional function was evaluated by sonomicrometry. Mean (+/- SEM) myocardial ATP concentration was 36.6 +/- 1.2 nmol/mg of cardiac protein in nonischemic subendocardium and 18.9 +/- 1.5 in ischemic subendocardium after 15 min of ischemia. ATP remained depressed in the reperfused previously ischemic subendocardium at both 90 min (68% of nonischemic value) and 72 hr (78% of nonischemic value) but returned to normal at 7 days. Regional systolic function and cardiac ultrastructural abnormalities required 7 days for full recovery. Histologic and histochemical analysis did not reveal necrosis at any time. Therefore, biochemical, functional, and ultrastructural abnormalities induced by brief periods of transient coronary occlusion not associated with necrosis do resolve completely but the recovery period is prolonged.
本研究的目的是确定短暂性冠状动脉闭塞（与坏死无关）所产生的生化、功能及超微结构异常是否会得到缓解，若会缓解，则确定何时缓解。对开胸麻醉的犬进行15分钟冠状动脉闭塞，随后再灌注72小时、7天或14天。进行系列活体心肌活检以测量ATP并进行超微结构分析。通过超声心动图评估局部功能。缺血15分钟后，非缺血性心内膜下心肌ATP浓度平均（±标准误）为36.6±1.2 nmol/mg心肌蛋白，缺血性心内膜下为18.9±1.5。再灌注后，先前缺血的心内膜下ATP在90分钟（非缺血值的68%）和72小时（非缺血值的78%）时仍处于降低状态，但在7天时恢复正常。局部收缩功能和心脏超微结构异常需要7天才能完全恢复。组织学和组织化学分析在任何时候均未显示坏死。因此，由短暂性冠状动脉闭塞（与坏死无关）引起的生化、功能及超微结构异常确实会完全缓解，但恢复期会延长。
