Reese R T, Motyl M R, Hofer-Warbinek R
Am J Trop Med Hyg. 1981 Nov;30(6):1168-78. doi: 10.4269/ajtmh.1981.30.1168.
Human and monkey sera from individuals exposed to Plasmodium falciparum were characterized by indirect immunofluorescence, in vitro parasite growth inhibition, and immunoprecipitation of 125I-labeled parasite antigens followed by analytical sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In general there was a good correlation between fluorescence titer and the ability of a serum to inhibit parasite growth in vitro. Exceptions were found, however. Some variance was seen in the ability of a given serum to inhibit different strains of the parasite. The significance of this is unknown. The proteins bound by human sera with high and low in vitro inhibitory capacities were compared by SDS-PAGE. The human sera which did not inhibit parasite growth in vitro well differed from those which did by failing to efficiently bind certain parasite components having molecular weights in the range of 200,000, 70,000-85,000, and 45,000.
采用间接免疫荧光、体外寄生虫生长抑制以及对125I标记的寄生虫抗原进行免疫沉淀后进行分析性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）的方法，对暴露于恶性疟原虫的人和猴血清进行了特性分析。总体而言，荧光滴度与血清在体外抑制寄生虫生长的能力之间存在良好的相关性。然而，也发现了一些例外情况。在给定血清抑制不同寄生虫菌株的能力方面存在一些差异。其意义尚不清楚。通过SDS-PAGE比较了具有高和低体外抑制能力的人血清所结合的蛋白质。在体外不能很好地抑制寄生虫生长的人血清与能抑制的血清不同，前者不能有效结合某些分子量在200,000、70,000 - 85,000和45,000范围内的寄生虫成分。
