Jensen J B, Boland M T, Allan J S, Carlin J M, Vande Waa J A, Divo A A, Akood M A
Infect Immun. 1983 Sep;41(3):1302-11. doi: 10.1128/iai.41.3.1302-1311.1983.
Clinical histories with regard to falciparum malaria were collected from adults living in holo-, hyper-, and hypoendemic areas of Sudan and matched to serum samples which were assayed for antiparasitic activity in cultures of Plasmodium falciparum. The adult population of the endemic areas could be divided into three groups based on oral histories: those who never experience falciparum malaria; those with a childhood history of malaria, who experience only mild occasional malaria as adults; and those who suffer serious recurring malaria symptoms. In vitro parasite inhibition was greatest with sera from individuals with no clinical histories of malaria, and generally, more inhibition was noted in sera from holoendemic versus hyperendemic areas. Serum from hypoendemic urban Khartoum was not inhibitory. There was no relationship between serum indirect fluorescent antibody titers and parasite inhibition, but there was strong association between clinical immunity and intraerythrocytic parasite inhibition resulting in "crisis" forms. Purified immunoglobulin G was not strongly associated with crisis forms, which were consistently associated with fractions of immune serum remaining after immunoglobulin removal. Thus, it appears that clinical immunity to malaria in Sudan is based on nonantibody serum factors, possibly associated with cell-mediated immunity. Human leukocyte alpha-interferon had no inhibitory effects on cultured P. falciparum. Some umbilical cord sera were profoundly inhibitory, producing crisis forms, whereas others were not inhibitory, suggesting that factors that induce crisis forms may play a role in protecting neonates from falciparum malaria.
收集了居住在苏丹疟疾全流行区、高流行区和低流行区的成年人的恶性疟临床病史，并将其与血清样本进行匹配，这些血清样本在恶性疟原虫培养物中检测了抗寄生虫活性。根据口述病史，流行区的成年人群可分为三组：从未患过恶性疟的人；有儿童疟疾史、成年后仅偶尔患轻度疟疾的人；以及患有严重复发性疟疾症状的人。没有疟疾临床病史的个体的血清对体外寄生虫的抑制作用最大，一般来说，全流行区血清的抑制作用比高流行区更明显。喀土穆低流行城市的血清没有抑制作用。血清间接荧光抗体滴度与寄生虫抑制之间没有关系，但临床免疫力与导致“危机”形式的红细胞内寄生虫抑制之间存在很强的关联。纯化的免疫球蛋白G与危机形式没有很强的关联，危机形式始终与去除免疫球蛋白后剩余的免疫血清成分有关。因此，苏丹对疟疾的临床免疫力似乎基于非抗体血清因子，可能与细胞介导的免疫有关。人白细胞α干扰素对培养的恶性疟原虫没有抑制作用。一些脐带血清具有很强的抑制作用，能产生危机形式，而其他血清则没有抑制作用，这表明诱导危机形式的因素可能在保护新生儿免受恶性疟方面发挥作用。
