抗体与单核细胞之间的合作可抑制恶性疟原虫的体外增殖。
Cooperation between antibodies and monocytes that inhibit in vitro proliferation of Plasmodium falciparum.
作者信息
Khusmith S, Druilhe P
出版信息
Infect Immun. 1983 Jul;41(1):219-23. doi: 10.1128/iai.41.1.219-223.1983.
Abstract
The cooperative effect between nonsensitized monocytes from normal individuals and malarial antibody in depressing parasite multiplication was investigated in an in vitro assay. The addition of purified normal monocytes to Plasmodium falciparum cultures in the presence of serum from immune individuals markedly inhibited the proliferation of the parasite in vitro: the parasitemia observed was about 22 times lower than that in the presence of immune serum alone. This cooperative effect was found to be effective over a wide range of monocyte/erythrocyte ratios (1:10 to 1:160) and serum concentrations (1/10 to 1/80). Immunoglobulin G extracted on protein A-Sepharose was as effective as total serum in this system. These data suggest that cooperation between nonsensitized cells and immunoglobulins could be an important effector mechanism against P. falciparum parasites in vivo.
摘要
在一项体外试验中，研究了正常个体未致敏单核细胞与疟疾抗体在抑制疟原虫增殖方面的协同作用。在来自免疫个体的血清存在下，将纯化的正常单核细胞添加到恶性疟原虫培养物中，可显著抑制体外寄生虫的增殖：观察到的疟原虫血症比仅存在免疫血清时低约22倍。发现这种协同作用在广泛的单核细胞/红细胞比例（1:10至1:160）和血清浓度（1/10至1/80）范围内均有效。在该系统中，用蛋白A-琼脂糖凝胶提取的免疫球蛋白G与全血清一样有效。这些数据表明，未致敏细胞与免疫球蛋白之间的协同作用可能是体内对抗恶性疟原虫的重要效应机制。
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