Tung J S, Scheid M P, Pierotti M A, Hämmerling U, Boyse E A
Immunogenetics. 1981;14(1-2):101-6. doi: 10.1007/BF00344303.
Conventional Ly-5 alloantisera precipitate cell-surface molecules of three sizes: 200K, 205K, and 220K. In SDS-PAGE, the rat monoclonal antibody 74/8' precipitates the same three molecules from both Ly-5.1 and Ly-5.2 cells. Cleveland mapping of the three molecules, precipitated by reaction of conventional Ly-5 alloantisera or 74/8' monoclonal antibody with lysates of 125I-labeled cells, disclosed no differences among the three molecular forms, but markedly distinguished all three Ly-5.1 molecules from all three Ly-5.2 molecules. Each of the three molecular forms can be expressed independently of the other two by cloned culture lines of Ly-5+ cells of different hematopoietic lineage. All of the seven cloned lines tested expressed only one form. However, two of the seven uncloned culture lines tested, plasmacytoma MOPC-70A and the X.1 putative macrophage line which originated from an SJL tumor, yielded both 200K and 205K forms.
传统的Ly-5同种抗血清能沉淀出三种大小的细胞表面分子：200K、205K和220K。在SDS-PAGE中，大鼠单克隆抗体74/8'能从Ly-5.1和Ly-5.2细胞中沉淀出相同的三种分子。通过传统Ly-5同种抗血清或74/8'单克隆抗体与125I标记细胞裂解物反应沉淀出的这三种分子进行Cleveland图谱分析，发现这三种分子形式之间没有差异，但能明显区分所有三种Ly-5.1分子和所有三种Ly-5.2分子。三种分子形式中的每一种都可以由不同造血谱系的Ly-5+细胞克隆培养系独立于其他两种进行表达。所测试的七个克隆系均只表达一种形式。然而，所测试的七个未克隆培养系中的两个，即浆细胞瘤MOPC-70A和源自SJL肿瘤的X.1假定巨噬细胞系，产生了200K和205K两种形式。
