Takata R, Aoyagi M, Mukai T
Mol Gen Genet. 1982;188(2):334-7. doi: 10.1007/BF00332697.
The gene for Escherichia coli ribosomal protein S15 (rpsO) was cloned on the vector pBR322 from F-prime JCH55 DNA. The recombinant plasmid was transformed to Serratia marcescens cells and it was proved that E. coli S15 was synthesized and incorporated into ribosome particles in S. marcescens cells. A DNA fragment containing rpsO was also inserted into the vector pRF3, which changes its copy number depending on the growth temperature in a temperature-sensitive polA host. By use of this recombinant plasmid it was shown that the relative synthesis rate of S15 increased about twice even when the copy number of the plasmid increased more than twenty-fold.
大肠杆菌核糖体蛋白S15（rpsO）基因从F-prime JCH55 DNA克隆到载体pBR322上。将重组质粒转化到粘质沙雷氏菌细胞中，结果证明大肠杆菌S15在粘质沙雷氏菌细胞中得以合成并整合到核糖体颗粒中。一个含rpsO的DNA片段也被插入到载体pRF3中，在温度敏感型polA宿主中，该载体的拷贝数会随生长温度而变化。利用这种重组质粒表明，即使质粒拷贝数增加了二十多倍，S15的相对合成速率也提高了约两倍。
