Stich H F, Rosin M P, Wu C H, Powrie W D
Mutat Res. 1981 Nov;90(3):201-12. doi: 10.1016/0165-1218(81)90001-x.
Chlorogenic acid, a compound which occurs naturally in many food items, was assayed for genotoxic activity in 3 different test systems: reverse mutations in the preincubation test with Salmonella typhimurium, gene conversion with Saccharomyces cerevisiae strain D7, and chromosome aberrations in Chinese hamster ovary (CHO) cells. Chlorogenic acid was directly convertogenic and clastogenic, but lacked a mutagenic capacity in the Salmonella bioassay. The transition metal Mn2+ enhanced the clastogenic and convertogenic activity of chlorogenic acid. In the presence of Mn2+ (10(-4)M), chlorogenic acid increased the frequency of his+ revertants in TA98 and TA100 strains of S. typhimurium. Caffeic acid and, to a lesser degree, quinic acid, which are components of chlorogenic acid, also showed genotoxic activity. The results show the importance of using several assays in combination with transition metals when testing for genotoxicity.
绿原酸是一种天然存在于许多食品中的化合物，在3种不同的测试系统中对其遗传毒性活性进行了测定：鼠伤寒沙门氏菌预孵育试验中的反向突变、酿酒酵母D7菌株的基因转换以及中国仓鼠卵巢（CHO）细胞中的染色体畸变。绿原酸具有直接的基因转换和断裂活性，但在沙门氏菌生物测定中缺乏诱变能力。过渡金属Mn2+增强了绿原酸的断裂和基因转换活性。在Mn2+（10(-4)M）存在的情况下，绿原酸增加了鼠伤寒沙门氏菌TA98和TA100菌株中his+回复突变体的频率。绿原酸的成分咖啡酸以及程度较轻的奎尼酸也表现出遗传毒性活性。结果表明，在测试遗传毒性时，将几种测定方法与过渡金属结合使用非常重要。
