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注射染料细胞的免疫细胞化学鉴定。

Immunocytochemical identification of dye-injected cells.

作者信息

Michaels R L

出版信息

J Histochem Cytochem. 1982 Feb;30(2):189-91. doi: 10.1177/30.2.7037940.

DOI:10.1177/30.2.7037940
PMID:7037940
Abstract

Lucifer Yellow CH may be injected into pancreatic islet cells and visualized in Epon sections of the embedded tissue both before and after plastic removal and immunocyto-chemical staining. The dye retains its fluorescence, clearly marking the injected cell and adjacent dye-coupled cells, but does not interfere with the indirect immunofluorescent staining patterns that are characteristic of the islet cells

摘要

路西法黄CH可注射到胰岛细胞中,并在包埋组织的环氧树脂切片中,在去除塑料和进行免疫细胞化学染色前后进行观察。该染料保留其荧光,清晰地标记注射的细胞和相邻的染料偶联细胞,但不干扰胰岛细胞特有的间接免疫荧光染色模式。

相似文献

1
Immunocytochemical identification of dye-injected cells.注射染料细胞的免疫细胞化学鉴定。
J Histochem Cytochem. 1982 Feb;30(2):189-91. doi: 10.1177/30.2.7037940.
2
Islets of Langerhans: dye coupling among immunocytochemically distinct cell types.胰岛:免疫细胞化学上不同细胞类型之间的染料偶联。
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Diabetes. 1986 Feb;35(2):232-6. doi: 10.2337/diab.35.2.232.
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Paraventricular neurones in the rat hypothalamic slice: Lucifer Yellow injection and immunocytochemical identification.大鼠下丘脑切片中的室旁神经元:荧光黄注射与免疫细胞化学鉴定
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Antiserum to lucifer yellow: preparation, characterization, and use for immunocytochemical localization of dye-filled retinal neurons.抗路西法黄抗血清:制备、特性鉴定及其用于染料填充视网膜神经元的免疫细胞化学定位
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引用本文的文献

1
Light microscopic immunocytochemical demonstration of peroxisomal enzymes in epon sections.环氧树脂切片中过氧化物酶体酶的光镜免疫细胞化学显示
Histochemistry. 1984;81(1):15-22. doi: 10.1007/BF00495395.
2
Junctions between lens fiber cells are labeled with a monoclonal antibody shown to be specific for MP26.晶状体纤维细胞之间的连接用一种单克隆抗体标记,该抗体已被证明对MP26具有特异性。
J Cell Biol. 1985 Jan;100(1):216-25. doi: 10.1083/jcb.100.1.216.
3
Properties of motor units in the transversus abdominis muscle of the garter snake.束带蛇腹横肌运动单位的特性
J Physiol. 1987 Dec;393:355-74. doi: 10.1113/jphysiol.1987.sp016827.