Meda P, Santos R M, Atwater I
Diabetes. 1986 Feb;35(2):232-6. doi: 10.2337/diab.35.2.232.
Cells found to be electrically active within microdissected mouse islets of Langerhans perifused with high (greater than or equal to 11.1 mM) glucose concentrations were labeled by injecting Lucifer yellow through the recording electrode. After fixation, these cells were located by fluorescence microscopy on sections serially cut throughout the islets and were subsequently identified by immunofluororescence staining with specific anti-islet hormone sera. Electrophysiologic control confirmed that the electrode tip had remained within the same cell throughout the experiment and showed that Lucifer yellow labeling did not affect the electrical activity of the impaled cell. Upon individual impalements, Lucifer yellow labeled either the impaled cell alone or this cell and some of its neighbors to which it was dye coupled. Immunofluorescence staining of the Lucifer yellow-labeled cells revealed that glucose-induced electrical activity was recorded from individual B-cells or groups of dye-coupled B-cells as well as from A-cells coupled to B-cells.
在灌注高（大于或等于11.1 mM）葡萄糖浓度的显微解剖小鼠胰岛中发现具有电活性的细胞，通过记录电极注射荧光黄进行标记。固定后，通过荧光显微镜在整个胰岛连续切片上定位这些细胞，随后用特异性抗胰岛激素血清进行免疫荧光染色鉴定。电生理对照证实，在整个实验过程中电极尖端始终位于同一个细胞内，并表明荧光黄标记不影响刺入细胞的电活动。在单个刺入时，荧光黄要么仅标记刺入的细胞，要么标记该细胞及其一些染料偶联的相邻细胞。对荧光黄标记细胞的免疫荧光染色显示，葡萄糖诱导的电活动是从单个B细胞或染料偶联B细胞群以及与B细胞偶联的A细胞中记录到的。
