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酵母2微米质粒DNA的体外复制：起源与方向。

Yeast 2-micrometer plasmid DNA replication in vitro: origin and direction.

作者信息

Kojo H, Greenberg B D, Sugino A

出版信息

Proc Natl Acad Sci U S A. 1981 Dec;78(12):7261-5. doi: 10.1073/pnas.78.12.7261.

DOI:10.1073/pnas.78.12.7261
PMID:7038673
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC349245/
Abstract

Most yeast strains harbor extrachromosomal 2-micrometer DNA, and this DNA synthesis, like nuclear DNA replication, is strictly under cell cycle control. A soluble extract of yeast Saccharomyces cerevisiae carries out semiconservative replication of added 2-micrometer DNA and Escherichia coli chimeric plasmids containing the 2-micrometer DNA. Replication is initiated on 10% of the DNA, and one round of replication is completed. The major products in early stages of replication are theta ("eye") forms which originate 140 +/- 50 nucleotides within one of the 599-base-pair inverted repeats of 2-micrometer DNA. Their replication is bidirectional and discontinuous. Extracts prepared from the cell division cycle mutant cdc8 show temperature-sensitive 2-micrometer DNA synthesis in vitro, suggesting that this in vitro system resembles in vivo 2-micrometer plasmid DNA replication. This system should provide a useful assay for the purification and characterization of yeast DNA replication proteins.

摘要

大多数酵母菌株都含有染色体外的2微米DNA，这种DNA的合成，如同核DNA复制一样，严格受细胞周期控制。酿酒酵母的一种可溶性提取物能对添加的2微米DNA以及含有2微米DNA的大肠杆菌嵌合质粒进行半保留复制。复制起始于10%的DNA，一轮复制完成。复制早期的主要产物是θ（“眼”）型，其起源于2微米DNA的599个碱基对反向重复序列之一内140±50个核苷酸处。它们的复制是双向且不连续的。从细胞分裂周期突变体cdc8制备的提取物在体外显示出对温度敏感的2微米DNA合成，这表明该体外系统类似于体内2微米质粒DNA的复制。该系统应为酵母DNA复制蛋白的纯化和特性鉴定提供一种有用的检测方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b40/349245/447e415c6024/pnas00663-0056-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b40/349245/80ed54dd4f85/pnas00663-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b40/349245/447e415c6024/pnas00663-0056-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b40/349245/80ed54dd4f85/pnas00663-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b40/349245/447e415c6024/pnas00663-0056-b.jpg

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本文引用的文献

1
Replication and recombination functions associated with the yeast plasmid, 2 mu circle.与酵母质粒2μm环相关的复制和重组功能。
Cell. 1980 Sep;21(2):501-8. doi: 10.1016/0092-8674(80)90487-0.
2
Genetic map of Saccharomyces cerevisiae.酿酒酵母的遗传图谱。
Microbiol Rev. 1980 Dec;44(4):519-71. doi: 10.1128/mr.44.4.519-571.1980.
3
Autonomously replicating sequences in Saccharomyces cerevisiae.酿酒酵母中的自主复制序列。
通过快速表达和增溶筛选选择用于结构测定的最佳真核生物整合膜蛋白。
J Mol Biol. 2009 Jan 23;385(3):820-30. doi: 10.1016/j.jmb.2008.11.021. Epub 2008 Nov 24.
4
Localization and sequence analysis of chloroplast DNA sequences of Chlamydomonas reinhardii that promote autonomous replication in yeast.莱茵衣藻叶绿体 DNA 序列在酵母中自主复制的定位和序列分析。
EMBO J. 1984 Feb;3(2):415-21. doi: 10.1002/j.1460-2075.1984.tb01822.x.
5
Model system for DNA replication of a plasmid DNA containing the autonomously replicating sequence from Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5399-403. doi: 10.1073/pnas.90.12.5399.
6
Protein complexes from active replicative fractions associate in vitro with the replication origins of yeast 2-micrometers DNA plasmid.来自活跃复制组分的蛋白质复合物在体外与酵母2微米DNA质粒的复制起点相关联。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3428-32. doi: 10.1073/pnas.79.11.3428.
7
In vitro replication of recombinant plasmids carrying chromosomal segments of Xenopus laevis.携带非洲爪蟾染色体片段的重组质粒的体外复制
Proc Natl Acad Sci U S A. 1982 Jun;79(12):3697-701. doi: 10.1073/pnas.79.12.3697.
8
New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication.酿酒酵母中影响DNA复制的新型温度敏感突变体。
Mol Gen Genet. 1982;187(1):42-6. doi: 10.1007/BF00384381.
9
DNA polymerases, deoxyribonucleases, and recombination during meiosis in Saccharomyces cerevisiae.酿酒酵母减数分裂过程中的DNA聚合酶、脱氧核糖核酸酶与重组
Mol Cell Biol. 1984 Dec;4(12):2811-7. doi: 10.1128/mcb.4.12.2811-2817.1984.
10
Saccharomyces cerevisiae CDC8 gene and its product.酿酒酵母CDC8基因及其产物。
Mol Cell Biol. 1984 Apr;4(4):583-90. doi: 10.1128/mcb.4.4.583-590.1984.
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6329-33. doi: 10.1073/pnas.77.11.6329.
4
Nucleotide sequence of the yeast plasmid.酵母质粒的核苷酸序列。
Nature. 1980 Aug 28;286(5776):860-5. doi: 10.1038/286860a0.
5
Discontinuous DNA replication.不连续DNA复制
Annu Rev Biochem. 1980;49:421-57. doi: 10.1146/annurev.bi.49.070180.002225.
6
Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.包含染色体复制起点和TRP1基因的酵母DNA片段的序列。
Gene. 1980 Jul;10(2):157-66. doi: 10.1016/0378-1119(80)90133-x.
7
Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis.酿酒酵母DNA合成起始过程中的顺序基因功能。
J Mol Biol. 1974 Apr 15;84(3):445-61. doi: 10.1016/0022-2836(74)90451-3.
8
Replicating circular DNA molecules in yeast.在酵母中复制环状DNA分子。
Cell. 1975 Mar;4(3):249-53. doi: 10.1016/0092-8674(75)90172-5.
9
Sequential function of gene products relative to DNA synthesis in the yeast cell cycle.酵母细胞周期中基因产物相对于DNA合成的顺序功能。
J Mol Biol. 1976 Jul 15;104(4):803-17. doi: 10.1016/0022-2836(76)90183-2.
10
Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.酵母中的转化：一种杂种克隆载体的构建及CAN1基因的分离
Gene. 1979 Dec;8(1):121-33. doi: 10.1016/0378-1119(79)90012-x.