Fekadu M, Chandler F W, Harrison A K
Arch Virol. 1982;71(2):109-26. doi: 10.1007/BF01314881.
Dogs were inoculated with either an Ethiopian of Mexican rabies virus strain. The distribution of viral antigen and lesions were studied by immunofluorescence, histologic and electron microscopic techniques. In all dogs inoculated with the Ethiopian rabies virus strain, tremendous whorls of filamentous fluorescing aggregates were observed throughout the brain; these were not observed in dogs inoculated with the Mexican virus. Lesions consisted on neuronal degeneration and neuronophagia, associated with large inclusion bodies and widespread inflammation in dogs inoculated with the Ethiopian isolate. All observed portions of the brain and spinal cord were affected. In general, lesions were much less severe with Mexican isolate. Occasional astrocytes were observed to have inclusions in dogs inoculated both with Ethiopian and Mexican strains. Most neurons examined electronmicroscopically showed signs of infection, varying from a small granular or finely fibrillar viral matrix to numerous matrices accompanied by prolific numbers of virus particles occupying much of the perikaryon. These were found in all dogs inoculated with the Ethiopian strain but were rare with the Mexican isolate. Viral budding occurred from membranes of the rough endoplasmic reticulum, outer lamella of the nuclear envelope, and rarely from the plasma membrane.
给狗接种了埃塞俄比亚或墨西哥狂犬病病毒株。通过免疫荧光、组织学和电子显微镜技术研究了病毒抗原的分布和病变情况。在所有接种埃塞俄比亚狂犬病病毒株的狗中，在整个大脑中都观察到巨大的丝状荧光聚集物漩涡；而在接种墨西哥病毒的狗中未观察到这种情况。病变包括神经元变性和噬神经元现象，在接种埃塞俄比亚分离株的狗中，还伴有大的包涵体和广泛的炎症。大脑和脊髓的所有观察部位均受到影响。总体而言，接种墨西哥分离株时病变要轻得多。在接种埃塞俄比亚和墨西哥毒株的狗中偶尔观察到星形胶质细胞有包涵体。大多数经电子显微镜检查的神经元显示出感染迹象，从少量颗粒状或细纤维状病毒基质到大量基质并伴有大量占据大部分核周质的病毒颗粒不等。这些在所有接种埃塞俄比亚毒株的狗中都能发现，但在接种墨西哥分离株的狗中很少见。病毒出芽发生在内质网的膜、核膜的外层，很少发生在质膜。
