att P7的染色体定位，即用于抑制大肠杆菌dnaA突变的不依赖recA的p7整合位点。
Chromosomal location of att P7, the recA-independent p7 integration site used in the suppression of Escherichia coli dnaA mutations.
作者信息
Chesney R H, Adler E
出版信息
J Bacteriol. 1982 Jun;150(3):1400-4. doi: 10.1128/jb.150.3.1400-1404.1982.
Abstract
Conjugational and transductional analyses were used to determine the chromosomal location of attP7, the recA-independent integration site used by bacteriophage P7 to suppress host dnaA mutations. The site of integration was found to be between tolC and dnaG. An increase in transduction frequencies was observed for markers surrounding attP7 when P7 was integrated. Under these conditions, all pairs of markers in this region, including those separated by attP7, were cotransduced at frequencies higher than normal, indicating the possible production of P7 specialized transducing particles.
摘要
采用接合分析和转导分析来确定attP7的染色体定位，attP7是噬菌体P7用于抑制宿主dnaA突变的不依赖recA的整合位点。发现整合位点位于tolC和dnaG之间。当P7整合时，观察到attP7周围标记的转导频率增加。在这些条件下，该区域内的所有标记对，包括那些被attP7隔开的标记对，共转导频率均高于正常水平，这表明可能产生了P7特异性转导颗粒。
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