Beaty B J, Casals J, Brown K L, Gundersen C B, Nelson D, McPherson J T, Thompson W H
J Clin Microbiol. 1982 Mar;15(3):429-34. doi: 10.1128/jcm.15.3.429-434.1982.
A clinically relevant indirect fluorescent-antibody technique (IFA) was developed for the serological diagnosis of La Crosse virus infections. The IFA (67%) was as sensitive as the hemagglutination inhibition (58%) and neutralization (58%) tests in the detection of antibodies in acute-phase specimens. Immunoglobulin M antibodies were detected by the IFA test in 48% (11 of 23) of these specimens. Diagnostically significant increases in IFA titer were detected in 86% (19 of 22) of the paired samples. Antibodies were detectable in some patients 7 years after infection; however, the IFA test was not as sensitive as the other two tests in the detection of previous infections.
已开发出一种临床相关的间接荧光抗体技术（IFA）用于拉克罗斯病毒感染的血清学诊断。在急性期标本中检测抗体时，IFA（67%）与血凝抑制试验（58%）和中和试验（58%）的敏感性相同。在这些标本中，48%（23份中的11份）通过IFA检测出免疫球蛋白M抗体。在86%（22对样本中的19对）的配对样本中检测到IFA滴度有诊断意义的升高。在感染7年后，仍可在一些患者中检测到抗体；然而，在检测既往感染时，IFA不如其他两种检测敏感。
