Boonpucknavig S, Vuttivirojana O, Siripont J, Futrakul P, Nimmannitya S
Am J Clin Pathol. 1975 Sep;64(3):365-71. doi: 10.1093/ajcp/64.3.365.
One hundred eighty-three sera from two groups of children with dengue hemorrhagic fever and 37 control sera were examined for antibody against dengue virus by an indirect immunofluorescent method. When the reciprocal titer of 16 or higher was used as the diagnostic level, positive tests could be obtained in 80% of Group I and 100% of Group II sera after 3--6 days of fever. Positive tests were obtained in 100% of both groups after the first week of fever. There was no false-positive among the control sera. Preparation of the antigen is relatively easy, and the antigen may be stored for at least 3 months. The immunofluorescent method is rapid and simple, and is recommended for routine detection of serum antibody in dengue hemorrhagic fever.
采用间接免疫荧光法检测了两组登革出血热患儿的183份血清及37份对照血清中的登革病毒抗体。以16或更高的效价作为诊断标准时，发热3至6天后，第一组80%的血清及第二组100%的血清检测呈阳性。发热第一周后两组血清检测均为100%阳性。对照血清中无假阳性。抗原制备相对容易，且抗原可保存至少3个月。免疫荧光法快速简便，推荐用于登革出血热血清抗体的常规检测。
