André C, Villeminot S, Bourhala S
Nouv Presse Med. 1982 Apr 3;11(15):1127-30.
Antibodies to double-stranded (native) DNA were detected in 886 sera by indirect immunofluorescence (IF) using Crithidia luciliae (CL) as substrate. Comparison with 208 sera tested by Farr's method showed that the IF-on-CL method was more sensitive and at least as specific to native DNA. Anti-native DNA antibodies were mostly found in patients with systemic lupus erythematosus. Complement-binding antibodies of this kind were detected in sera with high titres (greater than or equal to 1/100) of antibodies belonging to the G, A and M groups of immunoglobulins. The IF-on-CL test is easier, cheaper, at least as specific and probably more sensitive than radioimmunoassays. It makes it possible to identify the Ig group to which the anti-native DNA antibodies belong and their ability to bind the complement. It deserves to be used as routine test for the detection of these antibodies and for their repeated titration in the follow-up of patients with systemic lupus erythematosus.
以路氏锥虫（CL）为底物，通过间接免疫荧光法（IF）在886份血清中检测到双链（天然）DNA抗体。与采用Farr法检测的208份血清进行比较，结果表明基于CL的IF法对天然DNA更敏感且至少具有同等特异性。抗天然DNA抗体多见于系统性红斑狼疮患者。在免疫球蛋白G、A和M组抗体效价高（大于或等于1/100）的血清中检测到此类补体结合抗体。基于CL的IF检测比放射免疫分析更简便、成本更低，至少具有同等特异性且可能更敏感。它能够鉴定抗天然DNA抗体所属的免疫球蛋白类别及其结合补体的能力。它值得作为检测这些抗体以及在系统性红斑狼疮患者随访中对其进行重复滴定的常规检测方法。
