用二甲基亚硝胺处理的大鼠不同动力学类别的DNA中7-甲基鸟嘌呤和复制位点的分布。
Distribution of 7-methylguanine and of replication sites in the different kinetic classes of DNA from rats treated with dimethylnitrosamine.
作者信息
Galbraith A I, Chapleo M R, Itzhaki R F
出版信息
Nucleic Acids Res. 1978 Sep;5(9):3357-64. doi: 10.1093/nar/5.9.3357.
Abstract
The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be random between these classes of DNA. We have also studied chromatin DNA from rats treated with unlabelled DMN plus [3H]thymidine in this way, in order to find if DMN affects DNA synthesis within any one kinetic class. Our results suggest that there is no difference in the extent of synthesis between these classes.
摘要
利用DNA-DNA重缔合技术研究了大鼠肝脏染色质DNA重复序列和单一序列家族中7-甲基鸟嘌呤的分布情况。给大鼠注射二[¹⁴C]甲基亚硝胺，3小时后制备染色质DNA。发现7-甲基鸟嘌呤在这些DNA类别之间的分布是随机的。我们还以这种方式研究了用未标记的二甲基亚硝胺（DMN）加[³H]胸腺嘧啶处理的大鼠的染色质DNA，以确定DMN是否会影响任何一个动力学类别中的DNA合成。我们的结果表明，这些类别之间的合成程度没有差异。
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