The use of radioimmunoassay to study the formation and disappearance of O6-methylguanine in mouse liver satellite and main-band DNA following dimethylnitrosamine administration.

A sensitive radioimmunoassay for O6-methyldeoxyguanosine has been developed, permitting the analysis of microgram amounts of DNA. This technique has been used in the study of the formation and removal of O6-methylguanine in mouse liver satellite and main-band DNA. The results indicate a reduced extent of O6-methylguanine formation in satellite DNA but similar rates of its removal from both classes of DNA.