Ivanov S D, Kulikov S V
Biull Eksp Biol Med. 1982 Apr;93(4):37-9.
A simple, safe and rapid experimental scheme is offered for screening chemical compounds for ability to activate DNA reparation in bacterial cells. As DNA-damaging agent use was made of cell heating. The structural integrity of genome was determined during subsequent 90-minute incubation of bacteria. It was demonstrated that without additions to the culture medium, the cells are unable to recover the damaged genome, while addition of the compounds in question stimulated the reparation of bacterial DNA. p-Aminobenzoic acid (10(-5) M) was more powerful than aminobenzhydrazide as regards the recovery of genome integrity.
提供了一种简单、安全且快速的实验方案，用于筛选化合物激活细菌细胞中DNA修复的能力。使用细胞加热作为DNA损伤剂。在随后90分钟的细菌培养过程中测定基因组的结构完整性。结果表明，在不向培养基中添加任何物质的情况下，细胞无法修复受损的基因组，而添加相关化合物可刺激细菌DNA的修复。就基因组完整性的恢复而言，对氨基苯甲酸（10^(-5) M）比氨基苯肼更有效。
