Berggren A, Weiland A J, Ostrup L T
J Bone Joint Surg Am. 1982 Jul;64(6):799-809.
We studied the value of bone scintigraphy in the assessment of anastomotic patency and bone-cell viability in free bone grafts revascularized by microvascular anastomoses in twenty-seven dogs. The dogs were divided into three different groups, and scintigraphy was carried out using technetium-labeled methylene diphosphonate in composite bone grafts revascularized by microvascular anastomoses, conventional autogenous bone grafts, and periosteal grafts placed in different recipient beds. The viability of the grafts was evaluated by histological examination and fluorescence microscopy after triple labeling with oxytetracycline on the first postoperative day, alizarin complexone on the fourth postoperative day, and DCAF on the eleventh postoperative day. A positive scintiscan within the first week following surgery indicated patent microvascular anastomoses, and histological study and fluorescence microscopy confirmed that bone throughout the graft was viable. A positive scintiscan one week after surgery or later does not necessarily indicate microvascular patency or bone-cell survival, because new bone formed by creeping substitution on the surface of a dead bone graft can result in this finding.
我们研究了骨闪烁显像在评估27只狗中通过微血管吻合实现血管重建的游离骨移植中吻合口通畅性和骨细胞活力方面的价值。这些狗被分为三个不同的组，在通过微血管吻合实现血管重建的复合骨移植、传统自体骨移植以及置于不同受体床的骨膜移植中，使用锝标记的亚甲基二膦酸盐进行闪烁显像。在术后第一天用土霉素、术后第四天用茜素络合剂、术后第十一天用DCAF进行三重标记后，通过组织学检查和荧光显微镜评估移植物的活力。术后第一周内闪烁扫描呈阳性表明微血管吻合通畅，组织学研究和荧光显微镜证实整个移植物中的骨是有活力的。术后一周或更晚出现的阳性闪烁扫描不一定表明微血管通畅或骨细胞存活，因为在死骨移植表面通过爬行替代形成的新骨可能导致这一结果。
