Walker R A
J Pathol. 1982 Jun;137(2):109-17. doi: 10.1002/path.1711370204.
An indirect immunoperoxidase method has been used to study a series of breast carcinomas for the presence of five potential biological markers (two appropriate to breast, two placental proteins and one oncofetal protein). One or more markers were detectable in 84 per cent. of tumours, two or more in 60 per cent. and three of more in 32 per cent. Similarity of staining patterns was noted for two placental proteins and for the oncofetal protein and one placental protein. Markers appropriate to breast tissue tended to be detected in separate areas. In certain instances more than one antigen could be detected within the same cell. The relationship of the number of markers present to histological differentiation was found to be complex, but carcinomas with three or more antigens demonstrated showed a tendency to be well or moderately differentiated. Tumours with no markers often had not metastasised to lymph nodes but conversely those with four and five markers had. A striking throughout the study was the heterogenous secretory nature of breast carcinomas.
采用间接免疫过氧化物酶法研究了一系列乳腺癌中五种潜在生物标志物（两种与乳腺相关、两种胎盘蛋白和一种癌胚蛋白）的存在情况。在84%的肿瘤中可检测到一种或多种标志物，60%的肿瘤中可检测到两种或更多种标志物，32%的肿瘤中可检测到三种或更多种标志物。两种胎盘蛋白之间以及癌胚蛋白与一种胎盘蛋白之间的染色模式相似。与乳腺组织相关的标志物倾向于在不同区域被检测到。在某些情况下，同一细胞内可检测到不止一种抗原。发现存在的标志物数量与组织学分化之间的关系很复杂，但有三种或更多种抗原的癌显示出高分化或中分化的倾向。没有标志物的肿瘤通常尚未转移至淋巴结，但相反，有四种和五种标志物的肿瘤则已发生转移。整个研究中一个显著特点是乳腺癌的异质性分泌特性。
