Mizoguchi Y, Shiba T, Ohnishi F, Monna T, Yamamoto S, Nakai K, Otani S, Morisawa S
Hepatogastroenterology. 1981 Oct;28(5):254-7.
When peripheral blood lymphocytes from patients with various types of hepatitis were stimulated in vitro with liver specific protein, lymphocyte transformation and MIF production were demonstrated in many cases, especially in chronic active hepatitis. The culture supernatant of these activated lymphocytes was also shown to contain MAF, a kind of lymphokine, which activated the peritoneal macrophages of guinea pigs. When the culture fluid of MAF-activated macrophages was added to isolated liver cells, a significant inhibition of their albumin biosynthesis was detected. The active principle which caused the impairment of liver function was fractionated by gel filtration using a Sephadex G-75 column, and it was found that the active material was a protein-like substance with a molecular weight of about 10,000-40,000. The results suggest the possibility that the soluble substance released from the activated macrophage may be involved at least partially in the immunological pathogenesis of chronic active hepatitis among many other immunological processes.
用肝脏特异性蛋白在体外刺激各类肝炎患者的外周血淋巴细胞时，在许多病例中都出现了淋巴细胞转化及移动抑制因子（MIF）的产生，尤其是在慢性活动性肝炎患者中。这些活化淋巴细胞的培养上清液也被证明含有一种能激活豚鼠腹腔巨噬细胞的淋巴细胞因子——巨噬细胞激活因子（MAF）。当将MAF激活的巨噬细胞的培养液加入分离的肝细胞时，检测到其白蛋白生物合成受到显著抑制。通过使用葡聚糖凝胶G - 75柱进行凝胶过滤，对导致肝功能损害的活性成分进行了分离，结果发现活性物质是一种分子量约为10,000 - 40,000的类蛋白物质。这些结果提示，活化巨噬细胞释放的可溶性物质可能至少部分参与了慢性活动性肝炎在众多免疫过程中的免疫发病机制。
