Mizoguchi Y, Katoh H, Yamamoto S, Morisawa S
Gastroenterol Jpn. 1986 Feb;21(1):44-8. doi: 10.1007/BF02775939.
Liver cell damages were inducible, when isolated liver cells coated with specific antibody against the liver cell membrane were cultured with peripheral blood mononuclear cells. Although this antibody-dependent cell-mediated cytotoxicity (ADCC) was induced by the closed contact of effector cells to targets via the specific antibody, a cytotoxic factor or factors causing the inhibition of protein synthesis in the liver cells was detected in the culture supernatant of the ADCC reaction. Similarly, peritoneal exudate macrophages activated by endotoxin lipopolysaccharide also exerted cytotoxic effects on the isolated liver cells by producing a cytotoxic substance or substances. These liver cell injuries induced by either the ADCC or activated macrophage culture supernatant were significantly reduced, when the isolated liver cells were pretreated with tritoqualine before adding the cytotoxic culture supernatants. These results suggest that tritoqualine may protect liver cells from immunological injuries.
当用抗肝细胞膜的特异性抗体包被的分离肝细胞与外周血单个核细胞一起培养时，肝细胞损伤是可诱导的。尽管这种抗体依赖性细胞介导的细胞毒性（ADCC）是通过效应细胞经由特异性抗体与靶标的紧密接触诱导的，但在ADCC反应的培养上清液中检测到了一种或多种导致肝细胞蛋白质合成抑制的细胞毒性因子。同样，被内毒素脂多糖激活的腹腔渗出巨噬细胞也通过产生一种或多种细胞毒性物质对分离的肝细胞发挥细胞毒性作用。当在加入细胞毒性培养上清液之前用曲托喹啉预处理分离的肝细胞时，由ADCC或活化巨噬细胞培养上清液诱导的这些肝细胞损伤显著减轻。这些结果表明曲托喹啉可能保护肝细胞免受免疫损伤。
