Mizoguchi Y, Shiba T, Ohnishi F, Monna T, Yamamoto S, Morisawa S
Hepatogastroenterology. 1981 Oct;28(5):250-3.
The possible involvement of cell-mediated immune responses to liver-specific protein in the pathogenesis of liver injury was investigated. The subjects consisted of seven patients with acute hepatitis, 12 cases with chronic active hepatitis, four cases with chronic inactive hepatitis, and three cases with liver cirrhosis. When peripheral blood lymphocytes from these patients were cultured in the presence of liver specific protein, and lymphocyte transformation was determined by measuring the uptake of [3H]thymidine into acid-insoluble materials, positive blastogenesis was seen in two cases with acute hepatitis and in six cases with chronic active hepatitis. The macrophage activating factor (MAF), a kind of lymphokine, was also detectable in the culture medium of activated lymphocytes from six patients who showed positive blastogenesis by estimating [3H]glucosamine incorporation into macrophages. Furthermore, the MAF-activated macrophages wer shown to be cytotoxic for the isolated liver cells causing marked inhibition of albumin synthesis. This macrophage-mediated cytotoxicity was detected in eight cases that showed positive lymphocyte transformation. These observations suggest that macrophage-mediated cytotoxicity plays a role in the pathogenesis of chronic active hepatitis.
研究了细胞介导的针对肝脏特异性蛋白的免疫反应在肝损伤发病机制中的可能作用。研究对象包括7例急性肝炎患者、12例慢性活动性肝炎患者、4例慢性非活动性肝炎患者和3例肝硬化患者。当将这些患者的外周血淋巴细胞在肝脏特异性蛋白存在的情况下进行培养，并通过测量[3H]胸苷掺入酸不溶性物质来测定淋巴细胞转化时，在2例急性肝炎患者和6例慢性活动性肝炎患者中观察到阳性母细胞生成。通过估计[3H]葡糖胺掺入巨噬细胞，在6例显示阳性母细胞生成的患者的活化淋巴细胞培养基中也可检测到一种淋巴因子——巨噬细胞活化因子（MAF）。此外，MAF活化的巨噬细胞对分离的肝细胞具有细胞毒性，导致白蛋白合成受到明显抑制。在8例显示阳性淋巴细胞转化的病例中检测到这种巨噬细胞介导的细胞毒性。这些观察结果表明，巨噬细胞介导的细胞毒性在慢性活动性肝炎的发病机制中起作用。
