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3,3',5,5'-四甲基联苯胺在微孔板酶联免疫吸附测定中作为过氧化物酶底物的应用。

The use of 3,3',5,5'-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay.

作者信息

Goka A K, Farthing M J

出版信息

J Immunoassay. 1987;8(1):29-41. doi: 10.1080/01971528708063053.

DOI:10.1080/01971528708063053
PMID:3558827
Abstract

The assay conditions for the use of 3,3',5,5' tetramethylbenzidine (TMB) in microplate enzyme-linked immunosorbent assay are described. TMB is a safe (non-mutagenic) chromogen that is more sensitive than OPD as a substrate for horseradish peroxidase. We describe the optimum storage and assay conditions for this chromogen.

摘要

本文描述了在微孔板酶联免疫吸附测定中使用3,3',5,5'-四甲基联苯胺（TMB）的检测条件。TMB是一种安全（无致突变性）的显色剂，作为辣根过氧化物酶的底物，其比邻苯二胺更灵敏。我们描述了这种显色剂的最佳储存和检测条件。

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