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采用酶免疫分析法对尿液中的苯环利定进行定量分析。

Quantitation of phencyclidine in urine by enzyme immunoassay.

作者信息

Walberg C B, Gupta R C

出版信息

J Anal Toxicol. 1982 Mar-Apr;6(2):97-9. doi: 10.1093/jat/6.2.97.

DOI:10.1093/jat/6.2.97
PMID:7047902
Abstract

The EMIT phencyclidine assay was modified to permit quantitative results to be obtained on urine from patients intoxicated from ingestion of phencyclidine (PCP). Additional calibrators and a clinical processor for calculating the data are required. Precision and accuracy of the quantitative procedure was determined from urine spiked with PCP. The accuracy and specificity were determined by comparing the results obtained by the EMIT procedure with results obtained by gas chromatography. The procedure meets the requirements for a quantitative clinical assay. The short turnaround time of the assay makes it ideal for emergency room determination of PCP.

摘要

对酶放大免疫测定法(EMIT)苯环己哌啶检测方法进行了改进,以便能够对因摄入苯环己哌啶(PCP)而中毒患者的尿液获得定量结果。需要额外的校准物和用于计算数据的临床处理器。通过向尿液中添加PCP来确定定量程序的精密度和准确性。通过将EMIT程序获得的结果与气相色谱法获得的结果进行比较来确定准确性和特异性。该程序符合定量临床检测的要求。该检测方法周转时间短,使其成为急诊室检测PCP的理想选择。

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