鼠伤寒沙门氏菌尿嘧啶敏感突变体的分离与定位
Isolation and mapping of a uracil-sensitive mutant of Salmonella typhimurium.
作者信息
Bussey L B, Ingraham J L
出版信息
Mol Gen Genet. 1982;185(3):513-4. doi: 10.1007/BF00334151.
PMID:7048028
Abstract
A uracil-sensitive mutant of Salmonella typhimurium was isolated by diethyl sulfate mutagenesis and penicillin counterselection. This mutation identifies a new Salmonella gene that is well separated from the structural genes for arginine and pyrimidine biosynthesis. The use-1 mutation was located between the ilv gene cluster (isoleucine-valine operon) and hisR (structural gene for histidine tRNA) at 83 map units.
摘要
通过硫酸二乙酯诱变和青霉素反选择，分离出一株鼠伤寒沙门氏菌的尿嘧啶敏感突变体。该突变鉴定出一个新的沙门氏菌基因，它与精氨酸和嘧啶生物合成的结构基因相距甚远。use-1突变位于ilv基因簇（异亮氨酸-缬氨酸操纵子）和hisR（组氨酸tRNA的结构基因）之间，位于83个图距单位处。
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