O'Neill J P, Freundlich M
J Bacteriol. 1973 Oct;116(1):98-106. doi: 10.1128/jb.116.1.98-106.1973.
A strain of Salmonella typhimurium, MF1680, was isolated that required isoleucine for normal growth at 37 C but not at 27 C. In minimal medium the growth of MF1680 was strongly blocked by valine. Growth inhibition was reversed by isoleucine. When this mutant was grown at 37 C, the form of acetohydroxy acid synthetase not inhibited by valine, (AHAS(R)), could not be detected. The site of this mutation in AHAS(R), termed ilvG, was found to map in the ilv gene cluster between ilvD and ilvE.
分离出了一株鼠伤寒沙门氏菌MF1680，该菌株在37℃下正常生长需要异亮氨酸，但在27℃下则不需要。在基本培养基中，缬氨酸强烈抑制MF1680的生长。异亮氨酸可逆转生长抑制作用。当该突变体在37℃下生长时，无法检测到不受缬氨酸抑制的乙酰羟酸合成酶（AHAS(R)）形式。发现AHAS(R)中这个突变的位点（称为ilvG）位于ilvD和ilvE之间的ilv基因簇中。
