Williams G T, Hudson L
Parasitology. 1982 Jun;84(Pt 3):511-26. doi: 10.1017/s003118200005280x.
The design and operation of a modular, bacteriological continuous-flow culture system have been adapted for the growth of Trypanosoma cruzi epimastigotes in simple monophasic media. The system was designed to achieve a minimum of 200 days of continuous culture and provision was made for the continuous supply of medium and collection of parasites under sterile conditions. The system provides large quantities of epimastigotes with homogeneous morphology and uniform viability. The system also lends itself to the analysis of the factors which affect parasite growth. We examined the effects of changes in environmental parameters on epimastigote growth rate. Optimal growth was observed at 27 degrees C. The rate of stirring of the culture had a small but definable effect on the growth rate, which was greatest at 80 rp.m. Growth was only slightly affected by the level of dissolved oxygen between 10 and 50% saturation, but was inhibited at higher concentrations. Growth was slower at extreme values of pH but showed a broad optimum around pH 7.4.
一种模块化的细菌学连续流培养系统的设计与操作已适用于在简单单相培养基中培养克氏锥虫无鞭毛体。该系统旨在实现至少200天的连续培养，并配备了在无菌条件下连续供应培养基和收集寄生虫的装置。该系统可提供大量形态均匀、活力一致的无鞭毛体。该系统也有助于分析影响寄生虫生长的因素。我们研究了环境参数变化对无鞭毛体生长速率的影响。在27摄氏度时观察到最佳生长。培养物的搅拌速率对生长速率有微小但可确定的影响，在每分钟80转时最大。溶解氧水平在饱和度的10%至50%之间时，生长仅受到轻微影响，但在较高浓度时受到抑制。在pH值的极端值下生长较慢，但在pH 7.4左右显示出较宽的最佳范围。
