White G L, White G S
Am J Vet Res. 1982 Jun;43(6):1103-5.
A concentration of 1.4 X 10(9) Escherichia coli was added to tubes containing 10 ml of freshly collected blood from dogs to determine if prednisolone sodium succinate altered in vitro E coli mortality, glucose utilization, or neutrophil destruction. Group 1 tubes contained organisms plus saline solution, group 2 tubes had E coli plus 140 microgram of prednisolone/ml, and group 3 tubes contained E coli plus 1,400 microgram of prednisolone/ml. Initial and final WBC, RBC, PCV and E coli concentrations were determined on each group. Blood glucose values were measured from zero time through +2 hours. There was a significant (P less than 0.001) reduction in the number of E coli in vitro for all groups, but there was no difference among the groups. Fewer neutrophils (P less than 0.01) were lost in the tubes containing either concentration of prednisolone than in the tubes containing E coli alone. At the therapeutic amount of prednisolone (group 2) the glucose utilization was not significantly (P greater than 0.05) different from the utilization in the control group; however, at 10 X the therapeutic amount of prednisolone (group 3), there was an increase in glucose utilization. Seemingly, prednisolone does not alter in vitro E coli destruction and aids in preserving neutrophils of canine blood.
将浓度为1.4×10⁹的大肠杆菌添加到装有10毫升刚采集的犬血的试管中，以确定琥珀酸钠泼尼松龙是否会改变体外大肠杆菌的死亡率、葡萄糖利用率或中性粒细胞的破坏情况。第1组试管中含有细菌和盐溶液，第2组试管含有大肠杆菌加140微克泼尼松龙/毫升，第3组试管含有大肠杆菌加1400微克泼尼松龙/毫升。测定每组的初始和最终白细胞、红细胞、红细胞压积和大肠杆菌浓度。从零时间到2小时测量血糖值。所有组的体外大肠杆菌数量均有显著（P<0.001）减少，但各组之间无差异。与仅含有大肠杆菌的试管相比，含有任何一种浓度泼尼松龙的试管中损失的中性粒细胞更少（P<0.01）。在泼尼松龙的治疗剂量（第2组）下，葡萄糖利用率与对照组相比无显著差异（P>0.05）；然而，在泼尼松龙治疗剂量的10倍（第3组）下，葡萄糖利用率有所增加。似乎，泼尼松龙不会改变体外大肠杆菌的破坏情况，并有助于保护犬血中的中性粒细胞。
