Greaves M, Preston F E
Thromb Res. 1982 May 1;26(3):145-57. doi: 10.1016/0049-3848(82)90135-9.
A radioimmunoassay for 6-keto-prostaglandin F1alpha was developed and validated. The apparent concentration of 6-keto-prostaglandin F1alpha in human venous plasma was found to be less than 50 pg/m1. A rise in plasma 6-keto-prostaglandin F1alpha was detected on infusion of prostacyclin or of DDAVP in human subjects. Various methods of organic extraction were applied to plasma samples prior to radioimmunoassay. Organic extraction and silicic acid chromatography introduced marked and unacceptable nonspecific interference on subsequent radioimmunoassay. This interference was abolished by high pressure liquid chromatography of eluates and extracts prior to assay. Using these methods, a very low concentration of 6-ketoprostaglandin F1alpha in human venous plasma was confirmed.
开发并验证了一种用于检测6-酮-前列腺素F1α的放射免疫分析法。发现人静脉血浆中6-酮-前列腺素F1α的表观浓度低于50 pg/m1。在人体受试者中输注前列环素或去氨加压素后，检测到血浆6-酮-前列腺素F1α升高。在放射免疫分析之前，对血浆样本采用了各种有机萃取方法。有机萃取和硅酸色谱法对随后的放射免疫分析产生了明显且不可接受的非特异性干扰。通过在分析前对洗脱液和提取物进行高压液相色谱法，消除了这种干扰。使用这些方法，证实了人静脉血浆中6-酮-前列腺素F1α的浓度非常低。
