Illgen B
Z Parasitenkd. 1982;67(2):227-36. doi: 10.1007/BF00928118.
When fetal calf serum or human serum is added to the culture medium Tc 199, adult females of Litomosoides carinii release the same amount of microfilariae in vitro as after addition of cotton rat serum. In contrast, in media with serum of Meriones unguiculatus or Mastomys natalensis only a few microfilariae are released; moreover, addition of Mastomys serum immobilizes them. When males and females are kept together, the same amount of microfilariae is released as compared with single females. Worms 4 weeks of age (i.e., about 2 weeks before copulation) maintained in medium Tc 199 with 33% cotton rat serum do not mate (no insemination of the females). However, the females live 18 days (harmonic mean) in vitro and grow on average 5 mm in length (28%) and the males roll up their tails in characteristic manner. Worms 5 or 6 weeks of age kept in pairs for 45 to 6 days in vitro were implanted into cotton rats. In vivo they grew to normal length, copulated and delivered microfilariae into the circulating blood. Females implanted solely into the recipient grew as well but remained sterile; this means that no insemination took place in vitro.
当向培养基Tc 199中添加胎牛血清或人血清时，卡里尼丝虫的成年雌性在体外释放的微丝蚴数量与添加棉鼠血清后相同。相比之下，在含有长爪沙鼠或南非多乳鼠血清的培养基中，只有少量微丝蚴被释放；此外，添加南非多乳鼠血清会使它们固定不动。当雄性和雌性饲养在一起时，与单独的雌性相比，释放的微丝蚴数量相同。在含有33%棉鼠血清的培养基Tc 199中饲养4周龄（即交配前约2周）的蠕虫不会交配（雌性未受精）。然而，雌性在体外存活18天（调和平均数），平均体长增加5毫米（28%），雄性以特征性方式卷起尾巴。将5或6周龄的蠕虫成对在体外饲养45至6天，然后植入棉鼠体内。在体内，它们生长到正常长度，交配并将微丝蚴释放到循环血液中。仅植入受体的雌性生长良好，但仍不育；这意味着在体外未发生受精。
