Mössinger J, Wenk P
Z Parasitenkd. 1986;72(1):121-31. doi: 10.1007/BF00927743.
Several parameters concerning the reproduction of Litomosoides carinii were assessed using quantitatively infected cotton rats (Sigmodon hispidus). The course of embryogenesis from the fertilization of eggs to the delivery of the first microfilariae was observed by daily autopsies during prepatency. The duration of embryogenesis in vivo could thus be determined as 18 +/- 2 days. The contents of embryos in the uteri of female worms had been examined at various intervals. At the onset of patency 7-8 weeks p.i. the females were 71 +/- 6 mm long and on average contained 308 X 10(3) embryos/female, of which 19% were pathologically altered. In the middle of patency 16-20 weeks p.i. the females had grown up to 100 +/- 11 mm in length and now contained 509 X 10(3) embryos/female, 25% of them were pathologically altered, the others were normally developed. A positive correlation between the body length of a female worm and its number of embryos in utero was evident. Additionally the percentage of pathologically altered embryos was increased with respect to the age of the worms. The calculated fecundity of a female L. carinii in vivo of around 20 X 10(3) microfilariae/female per day had been confirmed with worms maintained in vitro. Three combinations of media and serum supplements were used and their influence on embryogenesis evaluated.
使用定量感染的棉鼠（棉鼠属）评估了与卡氏丝状线虫繁殖相关的几个参数。在潜伏期通过每日解剖观察从卵子受精到首批微丝蚴产出的胚胎发育过程。由此可确定体内胚胎发育持续时间为18±2天。在不同时间间隔检查了雌虫子宫内胚胎的情况。在感染后7 - 8周出现虫血症时，雌虫体长71±6毫米，平均每只雌虫含308×10³个胚胎，其中19%有病理改变。在感染后16 - 20周虫血症中期，雌虫体长增长至100±11毫米，此时每只雌虫含509×10³个胚胎，其中25%有病理改变，其余发育正常。雌虫体长与其子宫内胚胎数量之间存在明显的正相关。此外，病理改变胚胎的百分比随虫龄增加。计算得出卡氏丝状线虫雌虫在体内的繁殖力约为每天每只雌虫产出20×10³条微丝蚴，这在体外培养的虫体中得到了证实。使用了三种培养基和血清补充剂的组合，并评估了它们对胚胎发育的影响。
