Wenk P, Illgen B, Seitz H M
Z Parasitenkd. 1978 Mar 16;55(1):63-72. doi: 10.1007/BF00383477.
Embryos of L. carinii continue intrauterine development to microfilariae and are totally released into the medium within 5--6 days when the latter (Tc 199) is changed daily and air is used as the gas phase. Oogenesis or further fertilization of eggs, however, does not occur in vitro in any of the media examined by us. One female releases 140 X 10(3) microfilaria/day on an average in vitro within 5--6 days. Mean initial numbers of 300 X 10(3) Mf/female/day are observed. Addition of equine serum inhibits microfilarial release in vitro; normal cotton rat serum prolongs survival of females while total numbers of released microfilariae or retained embryonic stages are not increased. The serum of post-patent animals does not influence the numbers of released microfilariae or their viability or survival of females. Microfilariae released in vitro in Tc 199 + 33% normal cotton rat serum survive for more than 8 days, when air is used as the gas phase and the medium is changed daily. Microfilariae isolated from the blood of patent animals survive for at most 6 days, at a 48-hourly change of medium survival does not even exceed 4 days.
卡氏肺孢子虫胚胎在子宫内持续发育至微丝蚴，当每天更换后者（Tc 199）并以空气作为气相时，在5至6天内完全释放到培养基中。然而，在所检测的任何培养基中，卵母细胞发生或卵子进一步受精在体外均未发生。一只雌性在体外5至6天内平均每天释放140×10³条微丝蚴。观察到平均初始数量为300×10³条微丝蚴/雌性/天。添加马血清可抑制微丝蚴在体外的释放；正常棉鼠血清可延长雌性的存活时间，但释放的微丝蚴总数或保留的胚胎阶段数量并未增加。感染后动物的血清不影响释放的微丝蚴数量、其活力或雌性的存活。当以空气作为气相且每天更换培养基时，在Tc 199 + 33%正常棉鼠血清中体外释放的微丝蚴存活超过8天。从感染动物血液中分离的微丝蚴最多存活6天，当每48小时更换一次培养基时，存活甚至不超过4天。
