Roschger P, Hoerl E M, Stachelberger H, Plenk H
J Biomed Mater Res. 1980 Nov;14(6):765-76. doi: 10.1002/jbm.820140608.
A highly sensitive method to localize aluminum oxide or polyethylene wear particles in biological tissues is presented. Using the scanning electron microscope (SEM) with a special cathodoluminescence detector system, small amounts of these foreign materials can be visualized in simple frozen sections by their bright luminescence, even at low magnifications (200X). At higher magnifications, a differentiation of free as well as phagocytized aluminum oxide and polyethylene particles can be made due to their different behavior in light emission. In addition, aluminum oxide such as other metals can be identified by x-ray microprobe analysis. This combination of SEM with microprobe analysis and with the highly sensitive cathodoluminescence mode enables to detect and identify wear products from all the materials, commonly used in manufacturing artificial joint replacements.
本文介绍了一种在生物组织中定位氧化铝或聚乙烯磨损颗粒的高灵敏度方法。使用配备特殊阴极发光检测系统的扫描电子显微镜（SEM），即使在低放大倍数（200倍）下，也能通过其明亮的发光在简单的冷冻切片中观察到少量这些异物。在更高的放大倍数下，由于氧化铝和聚乙烯颗粒在发光方面的不同表现，可以区分游离的以及被吞噬的颗粒。此外，氧化铝与其他金属一样，可以通过X射线微探针分析来识别。SEM与微探针分析以及高灵敏度阴极发光模式的这种结合，能够检测和识别制造人工关节置换常用的所有材料产生的磨损产物。
