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鹦鹉热衣原体的表面特性。

Surface properties of Chlamydia psittaci.

作者信息

Vance D W, Hatch T P

出版信息

Infect Immun. 1980 Jul;29(1):175-80. doi: 10.1128/iai.29.1.175-180.1980.

Abstract

The surface properties of elementary bodies of Chlamydia psittaci were investigated by diethylaminoethyl-Sephadex chromatography, cytophoresis, partitioning in an aqueous polymer two-phase system, and hydrophobic interaction chromatography of the organism. The surface of C. psittaci was found to be hydrophobic and negatively charged at pH 7 and to have an isoelectric point of about pH 5. Reagents which block free carboxyl groups altered the surface charge of C. psittaci and caused the organism to agglomerate. The possible significance of hydrophobicity and surface charge on the ingestion of C. psittaci by host cells is discussed.

摘要

利用二乙氨基乙基葡聚糖凝胶色谱法、细胞电泳法、在水性聚合物双相系统中的分配以及该生物体的疏水相互作用色谱法,对鹦鹉热衣原体原体的表面特性进行了研究。发现鹦鹉热衣原体的表面在pH 7时具有疏水性且带负电荷,其等电点约为pH 5。封闭游离羧基的试剂改变了鹦鹉热衣原体的表面电荷,并导致该生物体聚集。文中讨论了疏水性和表面电荷对宿主细胞摄取鹦鹉热衣原体的可能意义。

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