Mahoney J R, Etkin N L, McSwigan J D, Eaton J W
Blood. 1982 Feb;59(2):439-42.
Abnormal erythrocyte Na+ transport has been reported in patients with essential hypertension and some first-degree relatives. The two major techniques now employed for estimating Na+ transport--Na+/Li+ countertransport and Na+/K+ cotransport--are rather intricate and time consuming. Furthermore, the precise nature of the transport processes being measured is not clear. We have developed a simpler, more direct technique based on measurement of 22Na+ accumulation by erythrocytes. 22Na+ uptake by red cells from patients with essential hypertension averages twice normal. Indeed, of 21 patients with essential hypertension, only 2 patients had values within the upper end of the normal range. In 12 patients with secondary hypertension and no family history of essential hypertension, erythrocyte 22Na+ accumulation was within normal limits. Control experiments indicate that our technique for estimating red cell 22Na+ uptake is highly reproducible and shows little day-to-day variation. This procedure for the assessment of erythrocyte Na+ transport should be useful in differential diagnosis and the presymptomatic identification of individuals genetically prone to essential hypertension.
据报道，原发性高血压患者及其一些一级亲属存在红细胞钠转运异常。目前用于评估钠转运的两种主要技术——钠/锂逆向转运和钠/钾协同转运——相当复杂且耗时。此外，所测量的转运过程的确切性质尚不清楚。我们基于测量红细胞对22Na+的摄取，开发了一种更简单、更直接的技术。原发性高血压患者红细胞对22Na+的摄取平均为正常水平的两倍。实际上，在21例原发性高血压患者中，只有2例的值在正常范围的上限之内。在12例继发性高血压且无原发性高血压家族史的患者中，红细胞22Na+的蓄积在正常范围内。对照实验表明，我们评估红细胞摄取22Na+的技术具有高度可重复性，且每日变化很小。这种评估红细胞钠转运的方法在鉴别诊断和对原发性高血压遗传易感性个体的症状前识别中应会有用。
