Pleban P A, Munyani A, Beachum J
Clin Chem. 1982 Feb;28(2):311-6.
We determined selenium concentrations and activities of the selenoenzyme, glutathione peroxidase (EC 1.11.1.9), in the plasma and erythrocytes of 38 apparently healthy women. We determined selenium concentrations directly by polarized Zeeman-effect flameless atomic absorption spectroscopy. Within-run precision studies for the assays gave CVs of 5.6% for a mean erythrocyte selenium concentration of 149.9 (SD 8.3) microgram/L (n = 10) and 6.4% for a mean plasma selenium concentration of 97.3 (SD 6.2) microgram/L (n = 12). For the women, mean selenium concentrations were 141.4 (SD 14.3) microgram/L of erythrocytes [0.49 (SD 0.07) microgram/g of hemoglobin and 96.3 (SD 14.2) microgram/L of plasma. Glutathione peroxidase activities were measured by a modification of the method of Paglia and Valentine (J. Lab. Clin. Med. 70: 158--169, 1967). Within-run precision studies for the glutathione peroxidase assays gave CVs of 12.8% for mean erythrocyte glutathione peroxidase activity of 77.2 (SD 9.9) U/g of hemoglobin (n = 13), and 8.1% for mean plasma activity of 312.5 (SD 25.2) U/L (n = 11). Mean enzyme activity was 78.7 (SD 12.9) U/g of hemoglobin for erythrocytes and 424 (SD 40) U/L for plasma. Erythrocyte selenium concentrations and glutathione peroxidase activities were positively, but poorly, correlated (r = 0.41, p less than 0.01).
我们测定了38名表面健康女性的血浆和红细胞中硒的浓度以及硒酶谷胱甘肽过氧化物酶（EC 1.11.1.9）的活性。我们通过偏振塞曼效应无火焰原子吸收光谱法直接测定硒浓度。分析的批内精密度研究表明，平均红细胞硒浓度为149.9（标准差8.3）微克/升（n = 10）时，变异系数为5.6%；平均血浆硒浓度为97.3（标准差6.2）微克/升（n = 12）时，变异系数为6.4%。这些女性的红细胞平均硒浓度为141.4（标准差14.3）微克/升[0.49（标准差0.07）微克/克血红蛋白]，血浆平均硒浓度为96.3（标准差14.2）微克/升。谷胱甘肽过氧化物酶活性通过对Paglia和Valentine方法（《临床检验杂志》70: 158 - 169, 1967）进行修改后进行测定。谷胱甘肽过氧化物酶分析的批内精密度研究表明，平均红细胞谷胱甘肽过氧化物酶活性为77.2（标准差9.9）单位/克血红蛋白（n = 13）时，变异系数为12.8%；平均血浆活性为312.5（标准差25.2）单位/升（n = 11）时，变异系数为8.1%。红细胞的平均酶活性为78.7（标准差12.9）单位/克血红蛋白，血浆为424（标准差40）单位/升。红细胞硒浓度与谷胱甘肽过氧化物酶活性呈正相关，但相关性较弱（r = 0.41，p < 0.01）。
