Mundy D E, Machin A F
J Chromatogr. 1982 Jan 15;234(2):427-35. doi: 10.1016/s0021-9673(00)81880-4.
The rodenticides brodifacoum, difenacoum, coumatetralyl and warfarin are determined in animal relicta by high-performance exclusion chromatography on porous silica. The first three compounds are not separated, but are subsequently differentiated by adsorption or reversed-phase high-performance liquid chromatography of the appropriate eluate fraction collected from the exclusion column. The method is rapid, and clean-up (on Sep-Pak silica cartridges) is simple. Mean recoveries from spiked substrates were generally above 80% at levels of 0.1-1.0 mg/kg. Routine limits of determination are about 0.05-0.1 mg/kg for warfarin and about 0.02 mg/kg for the other compounds. If analysis for warfarin is not required, the latter limit can be lowered to about 1 microgram/kg by a slight modification to the clean-up step.
采用多孔硅胶高效排阻色谱法测定动物残骸中的杀鼠剂溴敌隆、敌鼠隆、杀鼠迷和华法林。前三种化合物不能分离，但随后通过从排阻柱收集的适当洗脱液馏分的吸附或反相高效液相色谱法进行区分。该方法快速，净化（使用Sep-Pak硅胶柱）简单。在0.1 - 1.0 mg/kg的添加水平下，加标底物的平均回收率通常高于80%。华法林的常规测定限约为0.05 - 0.1 mg/kg，其他化合物约为0.02 mg/kg。如果不需要对华法林进行分析，通过对净化步骤稍作修改，后者的测定限可降至约1微克/千克。
