Habrioux G, Desfosses B, Condom R, Faure B, Jayle M
Steroids. 1978 Jul-Aug;32(1):61-71. doi: 10.1016/0039-128x(78)90100-9.
The simultaneous determinations of both 3alpha and 3beta epimers of 5alpha-androstane-3,17beta-diol as their glucuronides, sulfates and in their unconjugated forms are described. The diol estimation is carried out by radioimmunoassay with two specific immune sera after purification of the serum by use of chromatography on Sephadex LH-20. The values obtained (mean +/- S.D.) in pg/ml for the unconjugated 3alpha and 3beta epimers were, respectively, 267 +/- 67 and 816 +/- 76 for men; 114 +/- 33 and 515 +/- 177 for women; 142+/- 77 and 779 +/- 200 for hirsute women. Among the conjugates, the most important were the sulfoconjugates, their rates being, respectively (men +/- S.D. in ng/ml 41.6 +/- 9.5 and 103+/- 40 for men; 12.4 +/- 3.1 and 51.2 +/- 14.9 for women and 36 +/- 22 and 72 +/- 36 for hirsute women. Differences in the conjugation of both epimers were also noticed.
本文描述了同时测定5α-雄甾烷-3,17β-二醇的3α和3β差向异构体的葡萄糖醛酸苷、硫酸盐及其未结合形式。二醇的测定是通过放射免疫分析法，使用两种特异性免疫血清，在通过Sephadex LH-20柱色谱法纯化血清后进行。男性未结合的3α和3β差向异构体的测定值（pg/ml，平均值±标准差）分别为267±67和816±76；女性为114±33和515±177；多毛女性为142±77和779±200。在结合物中，最重要的是硫酸结合物，其含量（男性，ng/ml，平均值±标准差）分别为41.6±9.5和103±40；女性为12.4±3.1和51.2±14.9；多毛女性为36±22和72±36。还注意到两种差向异构体在结合方面的差异。
