Olveda R K, Andrews J S, Hewlett E L
Am J Trop Med Hyg. 1982 Jan;31(1):60-6. doi: 10.4269/ajtmh.1982.31.60.
A method was developed for quantitative localization of Giardia muris trophozoites in the small intestine of the mouse. Using this method, trophozoite distribution as a function of infecting dose of G. muris cysts, duration of infection, and mucosal histopathology was examined. Mice inoculated with 10 and 10(4) G. muris cysts were compared. The low dose group required longer to reach maximal levels of cyst excretion and trophozoite counts and had less marked histopathology. The groups were not different, however, in maximum rate of cyst excretion, maximum trophozoite count, and distribution of trophozoites and histopathology. The histopathological lesions were predominantly jejunal and transient, with beginning resolution despite continued presence of near maximal numbers of trophozoites on day 14. Early (day 4 and before) and late (day 90 and after) in the infection, cyst excretion was not a reliable indicator of the level of infection, since there were significant numbers of small bowel trophozoites in most animals without detectable cysts in the stool.
已开发出一种用于对小鼠小肠中鼠贾第虫滋养体进行定量定位的方法。使用该方法，研究了作为鼠贾第虫包囊感染剂量、感染持续时间和黏膜组织病理学函数的滋养体分布情况。比较了接种10个和10⁴个鼠贾第虫包囊的小鼠。低剂量组达到最大包囊排泄水平和滋养体计数所需时间更长，且组织病理学表现不那么明显。然而，两组在最大包囊排泄率、最大滋养体计数以及滋养体分布和组织病理学方面并无差异。组织病理学病变主要位于空肠且是短暂性的，尽管在第14天仍存在接近最大数量的滋养体，但病变已开始消退。在感染早期（第4天及之前）和晚期（第90天及之后），包囊排泄并非感染水平的可靠指标，因为大多数动物小肠中有大量滋养体，但粪便中未检测到包囊。
