Mower H F, Ichinotsubo D, Wang L W, Mandel M, Stemmermann G, Nomura A, Heilbrun L, Kamiyama S, Shimada A
Cancer Res. 1982 Mar;42(3):1164-9.
Human feces from 223 Japanese in Hawaii at high risk for colon cancer and feces from 166 Japanese of northern rural Japan at low risk for colon cancer were shown to contain mutagenic activity under five different test conditions. The first assay, using the Ames TA98 and TA100 Salmonella test, detected ether-soluble mutagens in the presence and absence of rat liver microsomes. Of these, the TA98 direct-acting mutagens are present more frequently in the feces of the high-risk population than the low-risk population at a high level of statistical significance (p less than 0.01). TA98 mutagens activated by rat liver microsomes also occur significantly more frequently in the feces of the Japanese from Hawaii (p less than 0.05). Mutagens detected by TA100 in the presence and absence of rat liver microsomes are not commonly found in either Japanese population. The second bacterial test system used to detect fecal mutagens uses Escherichia coli rec-. This system detects water-soluble fecal mutagens which are also present more frequently in the high-risk population than in the low-risk population (p less than 0.05).
对223名患结肠癌风险高的夏威夷日本人的粪便以及166名患结肠癌风险低的日本北部农村地区日本人的粪便，在五种不同测试条件下进行检测，结果显示均含有诱变活性。第一项检测采用Ames TA98和TA100沙门氏菌测试，在有和没有大鼠肝微粒体的情况下检测醚溶性诱变剂。其中，TA98直接作用诱变剂在高风险人群粪便中的出现频率显著高于低风险人群（p小于0.01）。由大鼠肝微粒体激活的TA98诱变剂在夏威夷日本人粪便中的出现频率也显著更高（p小于0.05）。在有和没有大鼠肝微粒体的情况下，TA100检测到的诱变剂在这两个日本人群体中都不常见。用于检测粪便诱变剂的第二个细菌测试系统使用大肠杆菌rec-。该系统检测到的水溶性粪便诱变剂在高风险人群中的出现频率也高于低风险人群（p小于0.05）。
