用带氮检测器的气液色谱法测定T氏和Blue氏成瘾者血液中的喷他佐辛和曲吡那敏。

Determination of pentazocine and tripelennamine in blood of T's and Blue addicts by gas-liquid chromatography with a nitrogen detector.

作者信息

Mackell M A, Poklis A

出版信息

J Chromatogr. 1982 Jan 29;235(2):445-52. doi: 10.1016/s0021-9673(00)85908-7.

Abstract

A procedure for the quantitative determination of pentazocine (T's) and tripelennamine (Blues) in blood obtained from T's and Blues addicts is described. The underivatized drugs were analyzed by gas-liquid chromatography with a nitrogen detector. The retention times relative to mepivicaine (internal standard) on OV-17 at 220 degrees C were: tripelennamine 0.69 and pentazocine 1.77. The linear ranges of blood standards were: tripelennamine, 0.10-1.00 microgram/ml; pentazocine, 0.50-5.0 microgram/ml. For simultaneous analysis, the within-run and between-run CVs of tripelennamine were 5.6% (n = 23) and 13% (n = 12); and for pentazocine 5.2% (n = 23) and 9.9% (n = 12). Mean recoveries over the range of standards were: tripelennamine, 103% +/- 2.5% (n = 12); pentazocine 77.8% +/- 3.6% (n = 12).

摘要

本文描述了一种用于定量测定从喷他佐辛（T's）和曲吡那敏（Blues）成瘾者采集的血液中喷他佐辛（T's）和曲吡那敏（Blues）含量的方法。未衍生化的药物通过带有氮检测器的气液色谱法进行分析。在220℃下，相对于甲哌卡因（内标）在OV - 17上的保留时间分别为：曲吡那敏0.69，喷他佐辛1.77。血液标准品的线性范围为：曲吡那敏，0.10 - 1.00微克/毫升；喷他佐辛，0.50 - 5.0微克/毫升。对于同时分析，曲吡那敏的批内和批间变异系数分别为5.6%（n = 23）和13%（n = 12）；喷他佐辛的批内和批间变异系数分别为5.2%（n = 23）和9.9%（n = 12）。标准品范围内的平均回收率为：曲吡那敏，103%±2.5%（n = 12）；喷他佐辛77.8%±3.6%（n = 12）。