一种从毫克级组织样本中定量提取酶和代谢物的方法。
A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range.
作者信息
Pette D, Reichmann H
出版信息
J Histochem Cytochem. 1982 Apr;30(4):401-2. doi: 10.1177/30.4.7061832.
PMID:7061832
Abstract
A method is described for quantitative extraction of soluble enzyme activities from tissue samples in the milligram range. Mechanical disintegration of the frozen tissue is performed in a micromortar from which the powdered tissue can be quantitatively transferred for extraction in small volumes within a microcentrifuge tube.
摘要
本文描述了一种从毫克级组织样本中定量提取可溶性酶活性的方法。将冷冻组织在微型研钵中进行机械破碎，破碎后的粉末状组织可定量转移至微量离心管中，用于小体积提取。
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