Meixensberger J, Herting B, Roggendorf W, Reichmann H
Department of Neurosurgery, University Würzburg, Germany.
J Neurooncol. 1995;24(2):153-61. doi: 10.1007/BF01078485.
Changes of mitochondrial and cytoplasm tumor metabolism were studied in malignant gliomas and normal cortex probes in vitro. By spectrophotometric methods marker enzymes of different mitochondrial (whole respiratory chain, citrate acid cycle, fatty oxidation) and cytoplasm (glycolysis, pentose phosphate shunt) metabolic energy pathways were analysed. Generally, the activities of intramitochondrial key enzymes were significantly decreased in gliomas when compared with enzyme activities of normal cortex tissue (p < 0.01). Glycolytic enzymes and a representative of the pentose phosphate shunt were unchanged or increased. Ratios of marker enzymes of the glycolytic pathway (lactate dehydrogenase) and glycose-6-P dehydrogenase revealed a significant difference between glioblastomas (p < 0.05) and grade III (p < 0.05) tumors in comparison to normal astrocytic tissue and astrocytomas WHO grade II. Thus, biochemical analyses allow metabolic grading of gliomas in vitro and may be a useful tool for understanding tumor biology.
在体外对恶性胶质瘤和正常皮质样本中线粒体和细胞质肿瘤代谢的变化进行了研究。通过分光光度法分析了不同线粒体（整个呼吸链、柠檬酸循环、脂肪酸氧化）和细胞质（糖酵解、磷酸戊糖途径）代谢能量途径的标记酶。总体而言，与正常皮质组织的酶活性相比，胶质瘤中线粒体内关键酶的活性显著降低（p < 0.01）。糖酵解酶和磷酸戊糖途径的一种代表性酶未发生变化或有所增加。与正常星形细胞组织和世界卫生组织二级星形细胞瘤相比，糖酵解途径标记酶（乳酸脱氢酶）和葡萄糖-6-磷酸脱氢酶的比率在胶质母细胞瘤（p < 0.05）和三级肿瘤（p < 0.05）之间存在显著差异。因此，生化分析能够在体外对胶质瘤进行代谢分级，可能是理解肿瘤生物学的有用工具。
